Design Perfect Padlock Probes
for miRNA Detection

Automated design pipeline for RCA-based CRISPR-Cas12a detection systems. Optimize specificity, stability, and efficiency in seconds.

Get Started View Documentation

$ python main.py --target_name "hsa-miR-21"

>>> Step 1: Initializing Sequence Pools...

>>> Step 2: Assembling Candidates...

[SUCCESS] Selection of 10 candidates is ready.

>>> Step 3: Running NCBI BLAST Validation...

[SUCCESS] CANDIDATE #1 IS VERIFIED AS SAFE.

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Why PadDx_miR?

Automated Design

Generates thousands of potential spacer and Cas12a core sequences instantly, optimized for your specific target miRNA.

Rigorous Filtering

Advanced algorithms filter out candidates with homopolymers, hairpins, and cross-hybridization risks to ensure high performance.

NCBI Validation

Integrated BLAST validation automatically checks candidates against the human transcriptome to prevent off-target effects.

Installation & Usage

1. Clone the Repository

git clone https://github.com/BKoo-Codes/PadDx_miR.git
cd PadDx_miR

2. Install Dependencies

pip install -r requirements.txt

3. Run the Tool

python main.py --target_name "hsa-miR-21-5p" \
               --target_seq "UAGCUUAUCAGACUGAUGUUGA"